Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

• Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing

• Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process

• Tested and found negative for bacteria, fungi, and mycoplasma

• Protein concentrations are determined by Lowry method

• Endotoxin units are measured by Limulus Amoebocyte Lysate assay

• BD Matrigel Matrix gel stability is tested for a period of 14 days at 37°C

• Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0mm layer of Corning Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor

Source
Engelbreth-Holm-Swarm Mouse Tumor

Application

• Cell Growth and Differentiation

• Metabolism/Toxicology Studies

• Invasion Assays

• in vitro and in vivo Angiogenesis Assays

• in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice

Features

• Certified LDEV-free

• Extensive quality control exceeding industry standard

• Rigorously validated for functionality

• Most extensively references basement membrane matrix

Click here for Corning Matrigel Lot Selection and Reservation.